tgf β1 Search Results


recombinant tgf β  (Miltenyi Biotec)
95
Miltenyi Biotec recombinant tgf β
( A and B ) Immunoblot analysis of TDRD3 in CD4 + YFP + T regs (A) or CD4 + cells (B) isolated from the spleen of indicated mice. Right: qPCR analysis of Tdrd3 mRNA ( n ≥ 3). ( C ) Representative flow cytometric analysis (left two panels), percentage (third panel) and number (right panel) of T reg (Foxp3 + ) cells in thymocytes from indicated mice ( n ≥ 6). ( D and E ) Representative flow cytometric analysis (left two panels) and percentage (third panel) of Foxp3 + iT regs among CD4 + T cells from Foxp3 YFP-Cre or Tdrd3 fl/fl /Foxp3 YFP-Cre mice (D) or from Tdrd3 fl/fl and Tdrd3 fl/fl /CD4 Cre mice (E), polarized in the presence <t>of</t> <t>TGF-β</t> (5 ng/ml) for 48 hours ( n ≥ 6 per treatment cohort). Foxp3 mRNA levels in differentiated iT regs were detected by qPCR (right). ( F ) Representative flow cytometric analysis (two left panels) and percentage (right panel) of live cells among Foxp3 + iT regs differentiated from naïve CD4 + T cells of indicated mice ( n ≥ 6). ( G ) Representative flow cytometric analysis (two left panels) and percentage (right panel) of the proliferating Foxp3 YFP-Cre or Tdrd3 fl/fl /Foxp3 YFP-Cre CD4 + T cells in indicated peaks shown on left, labeled with CellTrace Violet (CTV) and polarized under T reg conditions for 48 hours ( n ≥ 6). Boxed area: cell population of interest. Data are from three independent experiments [(A), (B), (C), (E), (F), and (G), right panels; presented as means ±SEM] or are from one representative of three independent experiments [(A), (B), (C), (E), (F), and (G), left panels]. ** P < 0.01, *** P < 0.001, and **** P < 0.0005; ns, not significant (two-tailed Student’s t test).
Recombinant Tgf β, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tgf+%CE%B21/pmc12829583-197-0-5?v=Miltenyi+Biotec
Average 95 stars, based on 1 article reviews
recombinant tgf β - by Bioz Stars, 2026-07
95/100 stars
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rat  (Multi Sciences (Lianke) Biotech Co Ltd)
97
Multi Sciences (Lianke) Biotech Co Ltd rat
( A and B ) Immunoblot analysis of TDRD3 in CD4 + YFP + T regs (A) or CD4 + cells (B) isolated from the spleen of indicated mice. Right: qPCR analysis of Tdrd3 mRNA ( n ≥ 3). ( C ) Representative flow cytometric analysis (left two panels), percentage (third panel) and number (right panel) of T reg (Foxp3 + ) cells in thymocytes from indicated mice ( n ≥ 6). ( D and E ) Representative flow cytometric analysis (left two panels) and percentage (third panel) of Foxp3 + iT regs among CD4 + T cells from Foxp3 YFP-Cre or Tdrd3 fl/fl /Foxp3 YFP-Cre mice (D) or from Tdrd3 fl/fl and Tdrd3 fl/fl /CD4 Cre mice (E), polarized in the presence <t>of</t> <t>TGF-β</t> (5 ng/ml) for 48 hours ( n ≥ 6 per treatment cohort). Foxp3 mRNA levels in differentiated iT regs were detected by qPCR (right). ( F ) Representative flow cytometric analysis (two left panels) and percentage (right panel) of live cells among Foxp3 + iT regs differentiated from naïve CD4 + T cells of indicated mice ( n ≥ 6). ( G ) Representative flow cytometric analysis (two left panels) and percentage (right panel) of the proliferating Foxp3 YFP-Cre or Tdrd3 fl/fl /Foxp3 YFP-Cre CD4 + T cells in indicated peaks shown on left, labeled with CellTrace Violet (CTV) and polarized under T reg conditions for 48 hours ( n ≥ 6). Boxed area: cell population of interest. Data are from three independent experiments [(A), (B), (C), (E), (F), and (G), right panels; presented as means ±SEM] or are from one representative of three independent experiments [(A), (B), (C), (E), (F), and (G), left panels]. ** P < 0.01, *** P < 0.001, and **** P < 0.0005; ns, not significant (two-tailed Student’s t test).
Rat, supplied by Multi Sciences (Lianke) Biotech Co Ltd, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tgf+%CE%B21/10__1016_slash_j__prmcm__2026__100800-99-31-57?v=Multi+Sciences+%28Lianke%29+Biotech+Co+Ltd
Average 97 stars, based on 1 article reviews
rat - by Bioz Stars, 2026-07
97/100 stars
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rabbit  (Servicebio Inc)
86
Servicebio Inc rabbit
( A and B ) Immunoblot analysis of TDRD3 in CD4 + YFP + T regs (A) or CD4 + cells (B) isolated from the spleen of indicated mice. Right: qPCR analysis of Tdrd3 mRNA ( n ≥ 3). ( C ) Representative flow cytometric analysis (left two panels), percentage (third panel) and number (right panel) of T reg (Foxp3 + ) cells in thymocytes from indicated mice ( n ≥ 6). ( D and E ) Representative flow cytometric analysis (left two panels) and percentage (third panel) of Foxp3 + iT regs among CD4 + T cells from Foxp3 YFP-Cre or Tdrd3 fl/fl /Foxp3 YFP-Cre mice (D) or from Tdrd3 fl/fl and Tdrd3 fl/fl /CD4 Cre mice (E), polarized in the presence <t>of</t> <t>TGF-β</t> (5 ng/ml) for 48 hours ( n ≥ 6 per treatment cohort). Foxp3 mRNA levels in differentiated iT regs were detected by qPCR (right). ( F ) Representative flow cytometric analysis (two left panels) and percentage (right panel) of live cells among Foxp3 + iT regs differentiated from naïve CD4 + T cells of indicated mice ( n ≥ 6). ( G ) Representative flow cytometric analysis (two left panels) and percentage (right panel) of the proliferating Foxp3 YFP-Cre or Tdrd3 fl/fl /Foxp3 YFP-Cre CD4 + T cells in indicated peaks shown on left, labeled with CellTrace Violet (CTV) and polarized under T reg conditions for 48 hours ( n ≥ 6). Boxed area: cell population of interest. Data are from three independent experiments [(A), (B), (C), (E), (F), and (G), right panels; presented as means ±SEM] or are from one representative of three independent experiments [(A), (B), (C), (E), (F), and (G), left panels]. ** P < 0.01, *** P < 0.001, and **** P < 0.0005; ns, not significant (two-tailed Student’s t test).
Rabbit, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tgf+%CE%B21/pm42107581-166-22-29?v=Servicebio+Inc
Average 86 stars, based on 1 article reviews
rabbit - by Bioz Stars, 2026-07
86/100 stars
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human tgf β1 elisa kit  (Elabscience Biotechnology)
93
Elabscience Biotechnology human tgf β1 elisa kit
( A and B ) Immunoblot analysis of TDRD3 in CD4 + YFP + T regs (A) or CD4 + cells (B) isolated from the spleen of indicated mice. Right: qPCR analysis of Tdrd3 mRNA ( n ≥ 3). ( C ) Representative flow cytometric analysis (left two panels), percentage (third panel) and number (right panel) of T reg (Foxp3 + ) cells in thymocytes from indicated mice ( n ≥ 6). ( D and E ) Representative flow cytometric analysis (left two panels) and percentage (third panel) of Foxp3 + iT regs among CD4 + T cells from Foxp3 YFP-Cre or Tdrd3 fl/fl /Foxp3 YFP-Cre mice (D) or from Tdrd3 fl/fl and Tdrd3 fl/fl /CD4 Cre mice (E), polarized in the presence <t>of</t> <t>TGF-β</t> (5 ng/ml) for 48 hours ( n ≥ 6 per treatment cohort). Foxp3 mRNA levels in differentiated iT regs were detected by qPCR (right). ( F ) Representative flow cytometric analysis (two left panels) and percentage (right panel) of live cells among Foxp3 + iT regs differentiated from naïve CD4 + T cells of indicated mice ( n ≥ 6). ( G ) Representative flow cytometric analysis (two left panels) and percentage (right panel) of the proliferating Foxp3 YFP-Cre or Tdrd3 fl/fl /Foxp3 YFP-Cre CD4 + T cells in indicated peaks shown on left, labeled with CellTrace Violet (CTV) and polarized under T reg conditions for 48 hours ( n ≥ 6). Boxed area: cell population of interest. Data are from three independent experiments [(A), (B), (C), (E), (F), and (G), right panels; presented as means ±SEM] or are from one representative of three independent experiments [(A), (B), (C), (E), (F), and (G), left panels]. ** P < 0.01, *** P < 0.001, and **** P < 0.0005; ns, not significant (two-tailed Student’s t test).
Human Tgf β1 Elisa Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tgf+%CE%B21/pmc11273830-165-25-29?v=Elabscience+Biotechnology
Average 93 stars, based on 1 article reviews
human tgf β1 elisa kit - by Bioz Stars, 2026-07
93/100 stars
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tgf β 1  (Revvity)
94
Revvity tgf β 1
( A and B ) Immunoblot analysis of TDRD3 in CD4 + YFP + T regs (A) or CD4 + cells (B) isolated from the spleen of indicated mice. Right: qPCR analysis of Tdrd3 mRNA ( n ≥ 3). ( C ) Representative flow cytometric analysis (left two panels), percentage (third panel) and number (right panel) of T reg (Foxp3 + ) cells in thymocytes from indicated mice ( n ≥ 6). ( D and E ) Representative flow cytometric analysis (left two panels) and percentage (third panel) of Foxp3 + iT regs among CD4 + T cells from Foxp3 YFP-Cre or Tdrd3 fl/fl /Foxp3 YFP-Cre mice (D) or from Tdrd3 fl/fl and Tdrd3 fl/fl /CD4 Cre mice (E), polarized in the presence <t>of</t> <t>TGF-β</t> (5 ng/ml) for 48 hours ( n ≥ 6 per treatment cohort). Foxp3 mRNA levels in differentiated iT regs were detected by qPCR (right). ( F ) Representative flow cytometric analysis (two left panels) and percentage (right panel) of live cells among Foxp3 + iT regs differentiated from naïve CD4 + T cells of indicated mice ( n ≥ 6). ( G ) Representative flow cytometric analysis (two left panels) and percentage (right panel) of the proliferating Foxp3 YFP-Cre or Tdrd3 fl/fl /Foxp3 YFP-Cre CD4 + T cells in indicated peaks shown on left, labeled with CellTrace Violet (CTV) and polarized under T reg conditions for 48 hours ( n ≥ 6). Boxed area: cell population of interest. Data are from three independent experiments [(A), (B), (C), (E), (F), and (G), right panels; presented as means ±SEM] or are from one representative of three independent experiments [(A), (B), (C), (E), (F), and (G), left panels]. ** P < 0.01, *** P < 0.001, and **** P < 0.0005; ns, not significant (two-tailed Student’s t test).
Tgf β 1, supplied by Revvity, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tgf+%CE%B21/pmc10405074-49-0-4?v=Revvity
Average 94 stars, based on 1 article reviews
tgf β 1 - by Bioz Stars, 2026-07
94/100 stars
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growth factor β tgfβ  (Proteintech)
96
Proteintech growth factor β tgfβ
( A and B ) Immunoblot analysis of TDRD3 in CD4 + YFP + T regs (A) or CD4 + cells (B) isolated from the spleen of indicated mice. Right: qPCR analysis of Tdrd3 mRNA ( n ≥ 3). ( C ) Representative flow cytometric analysis (left two panels), percentage (third panel) and number (right panel) of T reg (Foxp3 + ) cells in thymocytes from indicated mice ( n ≥ 6). ( D and E ) Representative flow cytometric analysis (left two panels) and percentage (third panel) of Foxp3 + iT regs among CD4 + T cells from Foxp3 YFP-Cre or Tdrd3 fl/fl /Foxp3 YFP-Cre mice (D) or from Tdrd3 fl/fl and Tdrd3 fl/fl /CD4 Cre mice (E), polarized in the presence <t>of</t> <t>TGF-β</t> (5 ng/ml) for 48 hours ( n ≥ 6 per treatment cohort). Foxp3 mRNA levels in differentiated iT regs were detected by qPCR (right). ( F ) Representative flow cytometric analysis (two left panels) and percentage (right panel) of live cells among Foxp3 + iT regs differentiated from naïve CD4 + T cells of indicated mice ( n ≥ 6). ( G ) Representative flow cytometric analysis (two left panels) and percentage (right panel) of the proliferating Foxp3 YFP-Cre or Tdrd3 fl/fl /Foxp3 YFP-Cre CD4 + T cells in indicated peaks shown on left, labeled with CellTrace Violet (CTV) and polarized under T reg conditions for 48 hours ( n ≥ 6). Boxed area: cell population of interest. Data are from three independent experiments [(A), (B), (C), (E), (F), and (G), right panels; presented as means ±SEM] or are from one representative of three independent experiments [(A), (B), (C), (E), (F), and (G), left panels]. ** P < 0.01, *** P < 0.001, and **** P < 0.0005; ns, not significant (two-tailed Student’s t test).
Growth Factor β Tgfβ, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tgf+%CE%B21/pm41917130-53-19-43?v=Proteintech
Average 96 stars, based on 1 article reviews
growth factor β tgfβ - by Bioz Stars, 2026-07
96/100 stars
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tgf β1  (Elabscience Biotechnology)
96
Elabscience Biotechnology tgf β1
( A and B ) Immunoblot analysis of TDRD3 in CD4 + YFP + T regs (A) or CD4 + cells (B) isolated from the spleen of indicated mice. Right: qPCR analysis of Tdrd3 mRNA ( n ≥ 3). ( C ) Representative flow cytometric analysis (left two panels), percentage (third panel) and number (right panel) of T reg (Foxp3 + ) cells in thymocytes from indicated mice ( n ≥ 6). ( D and E ) Representative flow cytometric analysis (left two panels) and percentage (third panel) of Foxp3 + iT regs among CD4 + T cells from Foxp3 YFP-Cre or Tdrd3 fl/fl /Foxp3 YFP-Cre mice (D) or from Tdrd3 fl/fl and Tdrd3 fl/fl /CD4 Cre mice (E), polarized in the presence <t>of</t> <t>TGF-β</t> (5 ng/ml) for 48 hours ( n ≥ 6 per treatment cohort). Foxp3 mRNA levels in differentiated iT regs were detected by qPCR (right). ( F ) Representative flow cytometric analysis (two left panels) and percentage (right panel) of live cells among Foxp3 + iT regs differentiated from naïve CD4 + T cells of indicated mice ( n ≥ 6). ( G ) Representative flow cytometric analysis (two left panels) and percentage (right panel) of the proliferating Foxp3 YFP-Cre or Tdrd3 fl/fl /Foxp3 YFP-Cre CD4 + T cells in indicated peaks shown on left, labeled with CellTrace Violet (CTV) and polarized under T reg conditions for 48 hours ( n ≥ 6). Boxed area: cell population of interest. Data are from three independent experiments [(A), (B), (C), (E), (F), and (G), right panels; presented as means ±SEM] or are from one representative of three independent experiments [(A), (B), (C), (E), (F), and (G), left panels]. ** P < 0.01, *** P < 0.001, and **** P < 0.0005; ns, not significant (two-tailed Student’s t test).
Tgf β1, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tgf+%CE%B21/10__34172_slash_jnp__2023__17363-89-1-4?v=Elabscience+Biotechnology
Average 96 stars, based on 1 article reviews
tgf β1 - by Bioz Stars, 2026-07
96/100 stars
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growth factor tgf β  (Cusabio)
93
Cusabio growth factor tgf β
( A and B ) Immunoblot analysis of TDRD3 in CD4 + YFP + T regs (A) or CD4 + cells (B) isolated from the spleen of indicated mice. Right: qPCR analysis of Tdrd3 mRNA ( n ≥ 3). ( C ) Representative flow cytometric analysis (left two panels), percentage (third panel) and number (right panel) of T reg (Foxp3 + ) cells in thymocytes from indicated mice ( n ≥ 6). ( D and E ) Representative flow cytometric analysis (left two panels) and percentage (third panel) of Foxp3 + iT regs among CD4 + T cells from Foxp3 YFP-Cre or Tdrd3 fl/fl /Foxp3 YFP-Cre mice (D) or from Tdrd3 fl/fl and Tdrd3 fl/fl /CD4 Cre mice (E), polarized in the presence <t>of</t> <t>TGF-β</t> (5 ng/ml) for 48 hours ( n ≥ 6 per treatment cohort). Foxp3 mRNA levels in differentiated iT regs were detected by qPCR (right). ( F ) Representative flow cytometric analysis (two left panels) and percentage (right panel) of live cells among Foxp3 + iT regs differentiated from naïve CD4 + T cells of indicated mice ( n ≥ 6). ( G ) Representative flow cytometric analysis (two left panels) and percentage (right panel) of the proliferating Foxp3 YFP-Cre or Tdrd3 fl/fl /Foxp3 YFP-Cre CD4 + T cells in indicated peaks shown on left, labeled with CellTrace Violet (CTV) and polarized under T reg conditions for 48 hours ( n ≥ 6). Boxed area: cell population of interest. Data are from three independent experiments [(A), (B), (C), (E), (F), and (G), right panels; presented as means ±SEM] or are from one representative of three independent experiments [(A), (B), (C), (E), (F), and (G), left panels]. ** P < 0.01, *** P < 0.001, and **** P < 0.0005; ns, not significant (two-tailed Student’s t test).
Growth Factor Tgf β, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tgf+%CE%B21/pm41887933-23-31-41?v=Cusabio
Average 93 stars, based on 1 article reviews
growth factor tgf β - by Bioz Stars, 2026-07
93/100 stars
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human tgf β1 assay  (Revvity)
90
Revvity human tgf β1 assay
( A and B ) Immunoblot analysis of TDRD3 in CD4 + YFP + T regs (A) or CD4 + cells (B) isolated from the spleen of indicated mice. Right: qPCR analysis of Tdrd3 mRNA ( n ≥ 3). ( C ) Representative flow cytometric analysis (left two panels), percentage (third panel) and number (right panel) of T reg (Foxp3 + ) cells in thymocytes from indicated mice ( n ≥ 6). ( D and E ) Representative flow cytometric analysis (left two panels) and percentage (third panel) of Foxp3 + iT regs among CD4 + T cells from Foxp3 YFP-Cre or Tdrd3 fl/fl /Foxp3 YFP-Cre mice (D) or from Tdrd3 fl/fl and Tdrd3 fl/fl /CD4 Cre mice (E), polarized in the presence <t>of</t> <t>TGF-β</t> (5 ng/ml) for 48 hours ( n ≥ 6 per treatment cohort). Foxp3 mRNA levels in differentiated iT regs were detected by qPCR (right). ( F ) Representative flow cytometric analysis (two left panels) and percentage (right panel) of live cells among Foxp3 + iT regs differentiated from naïve CD4 + T cells of indicated mice ( n ≥ 6). ( G ) Representative flow cytometric analysis (two left panels) and percentage (right panel) of the proliferating Foxp3 YFP-Cre or Tdrd3 fl/fl /Foxp3 YFP-Cre CD4 + T cells in indicated peaks shown on left, labeled with CellTrace Violet (CTV) and polarized under T reg conditions for 48 hours ( n ≥ 6). Boxed area: cell population of interest. Data are from three independent experiments [(A), (B), (C), (E), (F), and (G), right panels; presented as means ±SEM] or are from one representative of three independent experiments [(A), (B), (C), (E), (F), and (G), left panels]. ** P < 0.01, *** P < 0.001, and **** P < 0.0005; ns, not significant (two-tailed Student’s t test).
Human Tgf β1 Assay, supplied by Revvity, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tgf+%CE%B21/pmc07934936-183-16-20?v=Revvity
Average 90 stars, based on 1 article reviews
human tgf β1 assay - by Bioz Stars, 2026-07
90/100 stars
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mouse tgf β1  (Elabscience Biotechnology)
94
Elabscience Biotechnology mouse tgf β1
( A and B ) Immunoblot analysis of TDRD3 in CD4 + YFP + T regs (A) or CD4 + cells (B) isolated from the spleen of indicated mice. Right: qPCR analysis of Tdrd3 mRNA ( n ≥ 3). ( C ) Representative flow cytometric analysis (left two panels), percentage (third panel) and number (right panel) of T reg (Foxp3 + ) cells in thymocytes from indicated mice ( n ≥ 6). ( D and E ) Representative flow cytometric analysis (left two panels) and percentage (third panel) of Foxp3 + iT regs among CD4 + T cells from Foxp3 YFP-Cre or Tdrd3 fl/fl /Foxp3 YFP-Cre mice (D) or from Tdrd3 fl/fl and Tdrd3 fl/fl /CD4 Cre mice (E), polarized in the presence <t>of</t> <t>TGF-β</t> (5 ng/ml) for 48 hours ( n ≥ 6 per treatment cohort). Foxp3 mRNA levels in differentiated iT regs were detected by qPCR (right). ( F ) Representative flow cytometric analysis (two left panels) and percentage (right panel) of live cells among Foxp3 + iT regs differentiated from naïve CD4 + T cells of indicated mice ( n ≥ 6). ( G ) Representative flow cytometric analysis (two left panels) and percentage (right panel) of the proliferating Foxp3 YFP-Cre or Tdrd3 fl/fl /Foxp3 YFP-Cre CD4 + T cells in indicated peaks shown on left, labeled with CellTrace Violet (CTV) and polarized under T reg conditions for 48 hours ( n ≥ 6). Boxed area: cell population of interest. Data are from three independent experiments [(A), (B), (C), (E), (F), and (G), right panels; presented as means ±SEM] or are from one representative of three independent experiments [(A), (B), (C), (E), (F), and (G), left panels]. ** P < 0.01, *** P < 0.001, and **** P < 0.0005; ns, not significant (two-tailed Student’s t test).
Mouse Tgf β1, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tgf+%CE%B21/pmc09575261-217-2-12?v=Elabscience+Biotechnology
Average 94 stars, based on 1 article reviews
mouse tgf β1 - by Bioz Stars, 2026-07
94/100 stars
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tgf β  (Elabscience Biotechnology)
93
Elabscience Biotechnology tgf β
( A and B ) Immunoblot analysis of TDRD3 in CD4 + YFP + T regs (A) or CD4 + cells (B) isolated from the spleen of indicated mice. Right: qPCR analysis of Tdrd3 mRNA ( n ≥ 3). ( C ) Representative flow cytometric analysis (left two panels), percentage (third panel) and number (right panel) of T reg (Foxp3 + ) cells in thymocytes from indicated mice ( n ≥ 6). ( D and E ) Representative flow cytometric analysis (left two panels) and percentage (third panel) of Foxp3 + iT regs among CD4 + T cells from Foxp3 YFP-Cre or Tdrd3 fl/fl /Foxp3 YFP-Cre mice (D) or from Tdrd3 fl/fl and Tdrd3 fl/fl /CD4 Cre mice (E), polarized in the presence <t>of</t> <t>TGF-β</t> (5 ng/ml) for 48 hours ( n ≥ 6 per treatment cohort). Foxp3 mRNA levels in differentiated iT regs were detected by qPCR (right). ( F ) Representative flow cytometric analysis (two left panels) and percentage (right panel) of live cells among Foxp3 + iT regs differentiated from naïve CD4 + T cells of indicated mice ( n ≥ 6). ( G ) Representative flow cytometric analysis (two left panels) and percentage (right panel) of the proliferating Foxp3 YFP-Cre or Tdrd3 fl/fl /Foxp3 YFP-Cre CD4 + T cells in indicated peaks shown on left, labeled with CellTrace Violet (CTV) and polarized under T reg conditions for 48 hours ( n ≥ 6). Boxed area: cell population of interest. Data are from three independent experiments [(A), (B), (C), (E), (F), and (G), right panels; presented as means ±SEM] or are from one representative of three independent experiments [(A), (B), (C), (E), (F), and (G), left panels]. ** P < 0.01, *** P < 0.001, and **** P < 0.0005; ns, not significant (two-tailed Student’s t test).
Tgf β, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/tgf+%CE%B21/pm40384867-125-32-33?v=Elabscience+Biotechnology
Average 93 stars, based on 1 article reviews
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Image Search Results


( A and B ) Immunoblot analysis of TDRD3 in CD4 + YFP + T regs (A) or CD4 + cells (B) isolated from the spleen of indicated mice. Right: qPCR analysis of Tdrd3 mRNA ( n ≥ 3). ( C ) Representative flow cytometric analysis (left two panels), percentage (third panel) and number (right panel) of T reg (Foxp3 + ) cells in thymocytes from indicated mice ( n ≥ 6). ( D and E ) Representative flow cytometric analysis (left two panels) and percentage (third panel) of Foxp3 + iT regs among CD4 + T cells from Foxp3 YFP-Cre or Tdrd3 fl/fl /Foxp3 YFP-Cre mice (D) or from Tdrd3 fl/fl and Tdrd3 fl/fl /CD4 Cre mice (E), polarized in the presence of TGF-β (5 ng/ml) for 48 hours ( n ≥ 6 per treatment cohort). Foxp3 mRNA levels in differentiated iT regs were detected by qPCR (right). ( F ) Representative flow cytometric analysis (two left panels) and percentage (right panel) of live cells among Foxp3 + iT regs differentiated from naïve CD4 + T cells of indicated mice ( n ≥ 6). ( G ) Representative flow cytometric analysis (two left panels) and percentage (right panel) of the proliferating Foxp3 YFP-Cre or Tdrd3 fl/fl /Foxp3 YFP-Cre CD4 + T cells in indicated peaks shown on left, labeled with CellTrace Violet (CTV) and polarized under T reg conditions for 48 hours ( n ≥ 6). Boxed area: cell population of interest. Data are from three independent experiments [(A), (B), (C), (E), (F), and (G), right panels; presented as means ±SEM] or are from one representative of three independent experiments [(A), (B), (C), (E), (F), and (G), left panels]. ** P < 0.01, *** P < 0.001, and **** P < 0.0005; ns, not significant (two-tailed Student’s t test).

Journal: Science Advances

Article Title: TDRD3, a Tudor domain-containing protein, regulates Klf2 -dependent T reg differentiation and function to modulate immune tolerance

doi: 10.1126/sciadv.aea3960

Figure Lengend Snippet: ( A and B ) Immunoblot analysis of TDRD3 in CD4 + YFP + T regs (A) or CD4 + cells (B) isolated from the spleen of indicated mice. Right: qPCR analysis of Tdrd3 mRNA ( n ≥ 3). ( C ) Representative flow cytometric analysis (left two panels), percentage (third panel) and number (right panel) of T reg (Foxp3 + ) cells in thymocytes from indicated mice ( n ≥ 6). ( D and E ) Representative flow cytometric analysis (left two panels) and percentage (third panel) of Foxp3 + iT regs among CD4 + T cells from Foxp3 YFP-Cre or Tdrd3 fl/fl /Foxp3 YFP-Cre mice (D) or from Tdrd3 fl/fl and Tdrd3 fl/fl /CD4 Cre mice (E), polarized in the presence of TGF-β (5 ng/ml) for 48 hours ( n ≥ 6 per treatment cohort). Foxp3 mRNA levels in differentiated iT regs were detected by qPCR (right). ( F ) Representative flow cytometric analysis (two left panels) and percentage (right panel) of live cells among Foxp3 + iT regs differentiated from naïve CD4 + T cells of indicated mice ( n ≥ 6). ( G ) Representative flow cytometric analysis (two left panels) and percentage (right panel) of the proliferating Foxp3 YFP-Cre or Tdrd3 fl/fl /Foxp3 YFP-Cre CD4 + T cells in indicated peaks shown on left, labeled with CellTrace Violet (CTV) and polarized under T reg conditions for 48 hours ( n ≥ 6). Boxed area: cell population of interest. Data are from three independent experiments [(A), (B), (C), (E), (F), and (G), right panels; presented as means ±SEM] or are from one representative of three independent experiments [(A), (B), (C), (E), (F), and (G), left panels]. ** P < 0.01, *** P < 0.001, and **** P < 0.0005; ns, not significant (two-tailed Student’s t test).

Article Snippet: Recombinant TGF-β (#130-095-067) was from Miltenyi Biotec.

Techniques: Western Blot, Isolation, Labeling, Two Tailed Test